Lab 9/13: Isolation of Bacteria Part III

This week, we took a sample of our bacteria and did 2 things:

1) We made 2 stained slides by putting the bacteria on the slides and dropping 2 different color stains on it (Crystal Violet and Saffron).

2) We got an unknown sample (Sample C) and prepared an Agar Slant. The test tube said the bacteria should be incubated in 30 degrees Celsius, so we put it in the 30 degree Celsius incubator and we'll take it out on Thursday, The color of our sample was whitish yellowish.

For both parts of this lab, we used the  Aseptic Technique by sterilizing the loop every time we used it to transfer the bacteria to different petri dishes or containers, and we flamed the test tubes when transferring the unknown bacteria from the original test tube to the two others.

Lab 9/8: Isolation of Bacteria Part II

 (the top is the bacteria grown from the phone, the bottom is from the bathroom door handle)

This is the view of the bacteria using the microscope.

The left petri dish contains the 3 different types of bacteria, and one type was isolated and put in the petri dish on the right.

We took the petri dishes out of the incubator to observe the bacteria that grew since 9/4. The bacteria from the cell phone looked like white dots. There were 3 types of bacteria from the bathroom door handle. The first type had a concentric shape and filaments with a blue/green color and it was raised off the petri dish, the second type was circular and had a tan/cream color, the last one had a puntiform shape and also had tan/cream color. The next step of our project was to isolate a specific type of bacteria. In order to do this we got another petri dish and took a sample of the second type of bacteria and smeared it in different quadrants on the petri dish. It was then put in the incubator and will be taken out next week on  9/13.

Lab 9/6: Isolation of Bacteria Part 1

(the phone we used)

(the inside handle to the girl's bathroom)

The petri dish with the bacteria from the door handle's on the left and the one with bacteria from the phone is on the right.

This week in lab, we collected bacteria samples from various places. We swabbed a phone and the inside handle of the girls bathroom. In order to collect the bacteria, we took a test tube with broth and a swab, dipped the swab in the broth and wiped it on 1) the phone and 2) the bathroom door handle and then smeared each swab on 2 different petri dishes with Nutrient Agar Broth to grow the bacteria from both places. The petri dishes were put in an incubator, providing the ideal temperature for the bacteria to grow, and on Thursday, the petri dishes will be taken out and observed.

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