Lab 9/27: Gram Staining

We determined whether our Unknown Bacteria C was Gram Positive or Gram Negative. In order to do this we: 

1. Fixed a slide with a smear of bacteria

2. Stained the fixed slide with Crystal Violet Dye

3. Added Gram's iodine

4. 95% Ethanol was added as a de-colorizer solution which removed some of the stain

5. Added Safranin stain

Above are the fixed slides of our environmental bacteria (from the bathroom door) and our unknown bacteria.

The picture above is our unknown bacteria C

Above is the Gram stain of our Environmental Bacteria

Once the slide was stained, we looked at our bacteria using the oil immersion objective of the Compound Microscope, and determined that our bacteria is Gram Positive.